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Copyright of Journal of Atomic & Molecular Physics (1000-0364) is the property of Journal of Atomic & Molecular Physics Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

In order to analyze the safety risks of aircraft windshield crack / shatter events, 1 629 windshield crack / shatter events were collected, and the characteristics of the sample events involving conditions, flight phase, failure causes and other characteristics were statistically analyzed. The possible causes, potential consequences, prevention, mitigation and control measures of windshield crack / shatter events were determined, and the Bow-tie model of windshield crack / shatter events was drawn. The results show that the main conditions of windshield damage include crack, shatter, smoke, ice, scratch, etc. The main faults of windshield components are aircraft heating system and windshield wiper systems. The flight phase is mainly cruise and climb. The main causes of windshield crack / shatter events are bird strikes, parts missing / failure, system failure, ice strikes, and finally put forward targeted preventive measures, including maintenance, crew and other relevant personnel should do pre-flight and post-flight inspections; Focus on the types of high incidence incidents such as missing / damaged / worn parts, system failures, bird strikes, etc, and improve the response ability of the crew to such emergencies. [ABSTRACT FROM AUTHOR]

Copyright of Experimental Technology & Management is the property of Experimental Technology & Management Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Modern Educational Technology is the property of Editorial Board of Modern Educational Technology, Tsinghua University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of China Pulp & Paper is the property of China Pulp & Paper Magazines Publisher and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Atomic & Molecular Physics (1000-0364) is the property of Journal of Atomic & Molecular Physics Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Single crystal diamond has ultra-wide band gap, low dielectric constant, high breakdown voltage, high thermal conductivity, high intrinsic electron and hole mobility, and excellent radiation resistance, making it the most promising wide band gap high temperature semiconductor material known so far, known as the "ultimate semiconductor". However, there are still many technical problems to be solved in the large-scale application of single crystal diamond on semiconductors. This paper focuses on the chemical vapor deposition synthesis, stripping section and grinding and polishing technology of large size (inch) single crystal diamond substrates. By sorting out the relevant literature in recent years, the related research status at home and abroad are reviewed. On this basis, the preparation, stripping, grinding and polishing of single crystal diamond semiconductor materials in the future are prospected. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Chinese Mass Spectrometry Society is the property of Journal of Chinese Mass Spectrometry Society and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Atomic & Molecular Physics (1000-0364) is the property of Journal of Atomic & Molecular Physics Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Machine Tool & Hydraulics is the property of Guangzhou Mechanical Engineering Research Institute (GMERI) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To investigate the therapeutic effect and mechanism of bone marrow mesenchymal stem cell (BMSC) transplantation on early brain injury (EBI) in rats with subarachnoid hemorrhage (SAH) . Methods Forty-eight healthy male SD rats were randomly divided into the sham-operated group, SAH group, BMSC group and VitE group, with 10 rats in each group, and SAH models were constructed by intravascular puncture method. BMSCs were isolated, cultured, and identified. At 24 h after modeling, 3×106 BMSCs of the 4th generation were suspended in 0. 6 mL PBS and were injected into the BMSC group via the femoral vein； equal volume of PBS was injected into the sham-operated, SAH and VitE groups via the femoral vein. Vitamin E (100 mg/kg) was administered to the VitE group by gavage, and equal volume of olive oil was administered to the other groups by gavage. At 48 h after modeling, the neurological function of rats was assessed by modified Garcia score； the water content of brain tissues was calculated； the pathological changes of brain tissues were observed by HE staining； the number of necrotic neuronal cells was counted； the iron deposition in the brain tissues was detected by Prussian blue staining (iron staining) ； the iron content, MDA, and GSH in the brain tissues were detected by absorption photometry； the NOX2 protein in the brain tissues was detected by Western blotting, and ROS was detected by ROS probe method. Results Compared with the sham-operated group, the SAH group had lower modified Garcia score, higher brain tissue water content, lager number of necrotic nerve cells, higher brain tissue iron deposition, iron content, MDA content, lower GSH content, and higher NOX2 protein expression and higher ROS level in the brain tissues (all P< 0. 05) . Compared with the SAH group, the modified Garcia score was higher, the water content of brain tissue was lower, the necrotic nerve cells was less, the iron deposition, iron content and MDA content in the brain tissue were lower, the GSH content was higher, the expression of NOX2 protein and the level of ROS in brain tissues were lower in the BMSC group and VitE group (all P<0. 05) . Except for modified Garcia score, there were significant differences in the above indexes between BMSC group and VitE group (all P<0. 05) . The morphology of nerve cells in the sham-operated group was regular and uniform. In the SAH group, the nerve cells were arranged disorderly and the nucleus was broken. Compared with the SAH group, the injury of nerve cells in the BMSC group was significantly improved. The repair of nerve cell injury was better in the in BMSC group than in the VitE group. Conclusion BMSC transplantation can effectively treat EBI in SAH rats, and its mechanism of action may be the regulation of iron death through NOX2/ROS pathway. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Guangdong Architecture Civil Engineering is the property of Guangdong Architecture Civil Engineering Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Xinyang Normal University Natural Science Edition is the property of Journal of Xinyang Normal University Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Transactions of the Chinese Society of Agricultural Engineering is the property of Chinese Society of Agricultural Engineering and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of China Pulp & Paper is the property of China Pulp & Paper Magazines Publisher and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of China Pulp & Paper is the property of China Pulp & Paper Magazines Publisher and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Clean Coal Technology is the property of Clean Coal Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective：To construct the prokaryotic expression plasmid of transcription factor EB(TFEB) serine 114 site mutant based on gene splicing by overlap extension PCR (SOE PCR)technique, and to induce the expression and purification in vitro. Methods：The mutant primers were designed according to the principle of SOE PCR technique. First, the pGEX-6p-1-TFEB plasmid was used as the template, outer primers F and R and mutation primers Fn and Rn were used for the first step PCR amplification to obtain product 1 and product 2 containing mutation sites. Secondly, overlap splicing of product 1 and product 2 was performed by annealing and extension of the second step PCR. Lastly, using the spliced DNA fragment as a template, the third step PCR amplification was performed to obtain the target DNA containing the mutation sites by using outer primers F and R. Then the target DNA fragments were cloned into the pGEX-6p-1 vector, which were identified by enzyme digestion with BamH Ⅰ and Sal Ⅰ . The mutation results were verified by DNA sequencing. The expressions of TFEB and its mutant recombinant proteins were induced in Escherichia coli(E. coli) with different concentrations of isopropyl-beta-Dthiogalactopyranoside( IPTG)and different conditions. The purified proteins were purified by Glutathione- Sepharose 4B agarose beads, and the protein concentrations and molecular weights of the purified products were detected by SDS-PAGE gel electrophoresis. Results：Two DNA fragments containing mutation sites were obtained by the first step of PCR amplification, and a large number of complete DNA fragments containing mutation sites were obtained after the annealing and extension of the second step of PCR and the amplification of the third step of PCR. The double-enzyme digestion identification results showed that the size of the ligated DNA fragment was the same as that of the target fragment. The DNA sequencing results showed that the 114-position serine(TCT)of TFEB was successfully mutated to alanine(GCT). The soluble protein expression of TFEB and its mutants were successfully obtained under the condition of 0. 5 mmol·L－1 IPTG and overnight at 16 ℃. The results of SDS-PAGE gel electrophoresis showed that the purified protein concentration was higher and the molecular size was correct. Conclusion：The site-directed mutagenesis of serine 114 of TFEB is successfully achieved by SOE PCR technique, and TFEB and its mutant genes are successfully expressed in E. coli. [ABSTRACT FROM AUTHOR]

Copyright of Acta Edulis Fungi is the property of Acta Edulis Fungi and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Railway Science & Engineering is the property of Journal of Railway Science & Engineering Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Natural Gas Geoscience is the property of Natural Gas Geoscience and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Shenyang Agricultural University is the property of Journal of Shenyang Agricultural University Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of China Pulp & Paper is the property of China Pulp & Paper Magazines Publisher and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Yingyong Shengtai Xuebao is the property of Chinese Journal of Applied Ecology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Control Theory & Applications / Kongzhi Lilun Yu Yinyong is the property of Editorial Department of Control Theory & Applications and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Acta Materiae Compositae Sinica is the property of Acta Materiea Compositae Sinica Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Experimental Technology & Management is the property of Experimental Technology & Management Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Transactions of China Pulp & Paper is the property of China Pulp & Paper Magazines Publisher and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objectives: The extraction of boundary points and boundary lines is a very important work in land ownership confirmation. The traditional boundary points and boundary lines extraction method first needs to be judged manually, and then the attribute information corresponding to the boundary points and boundary lines is manually input into the attribute table according to the result of judgment. Excessive manual intervention not only makes the work inefficient, but also easy to cause all kinds of errors.Methods: In or-der to solve the above problems, an optimization algorithm for boundary points and boundary lines extrac-tion is proposed. The idea of the algorithm is as follows: (1) Using the modeling tools in ArcGIS to estab-lish a set of data preprocessing model for all parcels, and generate the template files of boundary points and boundary lines through the model; (2) Read some attribute field information of parcel and template file by using GDAL (geographic data abstraction library); (3) Generate and automatically read parcel boundary points and boundary lines file.Results: The experimental results show that this method can effectively avoid human intervention and greatly improve the extraction efficiency of boundary points and boundary lines. Conclusions: In the actual production, especially in the extraction of large quantities of boundary points and lines, this method has considerable advantages and provides original technical support for similar land moni-toring and management in China [ABSTRACT FROM AUTHOR]

Copyright of Journal of Jilin University (Science Edition) / Jilin Daxue Xuebao (Lixue Ban) is the property of Zhongguo Xue shu qi Kan (Guang Pan Ban) Dian zi Za zhi She and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of National University of Defense Technology / Guofang Keji Daxue Xuebao is the property of NUDT Press and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Nonferrous Metals (Mining Section) is the property of Beijing Research Institute of Mining & Metallurgy Technology Group and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Acta Edulis Fungi is the property of Acta Edulis Fungi and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Mycosystema is the property of Mycosystema Editorial Board and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)